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STEMCELL Technologies Inc rosetteseptm human b cell enrichment cocktail
Rosetteseptm Human B Cell Enrichment Cocktail, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rosetteseptm+human+b+cell+enrichment+cocktail/rosettesep+human+b+cell+enrichment+cocktail/pmc11331101__pnas__2405644121__sapp-39-12-22
Average 90 stars, based on 1 article reviews
rosetteseptm human b cell enrichment cocktail - by Bioz Stars, 2026-09
90/100 stars

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Purification:

Article Title: Measurable residual disease study through three different methods can anticipate relapse and guide early interventions in childhood acute lymphoblastic leukemia.
Article Snippet: Introduction Acute lymphoblastic leukemia (ALL) is the most common cancer among children.. Measurable residual disease (MRD, previously named minimal residual disease) study can guide therapy adjustments or preemptive interventions that might avoid hematological relapse.. Methods Clinical decision making and patient outcome were evaluated in 80 real-life childhood ALL patients, according to the results observed in 544 bone marrow samples analyzed with three MRD methods: multiparametric flow cytometry (MFC), fluorescent in-situ hybridization (FISH) on B or T-purified lymphocytes and patient-specific nested reverse transcription polymerase chain reaction (RT-PCR).

Article Title: Supporting Information for IL-1 RECEPTOR ANTAGONISM REVEALS A YIN-YANG RELATIONSHIP BETWEEN NF B- AND INTERFERON-SIGNALING IN CHRONIC LYMPHOCYTIC LEUKEMIA
Article Snippet: .. The cells were purified as before (4, 5) by negative selection using RosetteSepTM Human B cell enrichment Cocktail (catalog. no. 15064) from Stemcell technologies (Vancouver, BC). ..

Incubation:

Article Title: Monoclonal antibodies and cocktails for treatment of Ebola infections
Article Snippet: B Cell and Plasma Isolation Approximately 85 ml of whole blood was collected in 8.5 ml ACD Solution A VACUTAINER® venous blood collection tubes (Becton Dickinson) per the manufacturer's protocol. .. Blood was transported at room temperature and distributed into 50 ml conical tubes before addition of 300 μl of ROSETTESEPTM human B cell enrichment cocktail (StemCell Technologies) per 21 ml of blood, mixed by inversion and incubated for 20 minutes at room temperature. .. The total volume was brought to 50 ml with Hank's Balanced Salt Solution (HBSS), layered over Ficoll-Paque Plus (GE Healthcare) and centrifuged following the manufacturer's protocol.

Isolation:

Article Title: B Cells Isolated from Individuals Who Do Not Respond to the HBV Vaccine Are Characterized by Higher DNA Methylation-Estimated Aging Compared to Responders.
Article Snippet: .. PBMC samples were collected from the recruited vaccinated subjects and B cells were isolated by negative selection using the RosetteSepTM Human B Cell Enrichment Cocktail (Stemcell Technologies, Vancouver, BC, Canada). .. Genomic DNA was extracted with QIAamp DNA Blood Mini Kit (Qiagen, Venlo, The Netherlands) following the manufacturer’s “Blood or Body Fluid Spin Protocol”, using for final elution 130 μL of Buffer AE.

Article Title: Development of KoRV-pseudotyped lentiviral vectors for efficient gene transfer into freshly isolated immune cells
Article Snippet: .. Cells were then isolated by standard density-gradient centrifugation using Ficoll-Paque (VWR, Germany, Catalog. Nr. 17-1440-03) using the RosetteSepTM Human NK Cell Enrichment Cocktail (Stemcell Technologies, Canada) for NK cells, the RosetteSepTM Human B Cell Enrichment Cocktail for B cells and the RosetteSepTM Human Monocyte Enrichment Cocktail for monocytes, respectively. .. NK cells were cultured at 1 × 10 6 cells/mL in NK MACS medium (Miltenyi Biotec, Germany) with 5% human AB serum (Sigma Aldrich, USA, Catalog Nr. H4522-100ML), 500 U/mL IL-2 and 140 U/mL IL-15 (Peprotech, USA).

Article Title: Development of KoRV-pseudotyped lentiviral vectors for efficient gene transfer into freshly isolated immune cells.
Article Snippet: .. Cells were then isolated by standard density- gradient centrifugation using Ficoll-Paque (VWR, Germany, Catalog. Nr. 17- 1440-03) using the RosetteSepTM Human NK Cell Enrichment Cocktail (Stemcell Technologies, Canada) for NK cells, the RosetteSepTM Human B Cell Enrichment Cocktail for B cells and the RosetteSepTM Human Monocyte Enrichment Cocktail for monocytes, respectively. .. NK cells were cultured at 1 × 106 cells/mL in NK MACS medium (Miltenyi Biotec, Germany) with 5% human AB serum (Sigma Aldrich, USA, Catalog Nr. H4522-100ML), 500 U/mL IL-2 and 140 U/mL IL-15 (Peprotech, USA).

Selection:

Article Title: B Cells Isolated from Individuals Who Do Not Respond to the HBV Vaccine Are Characterized by Higher DNA Methylation-Estimated Aging Compared to Responders.
Article Snippet: .. PBMC samples were collected from the recruited vaccinated subjects and B cells were isolated by negative selection using the RosetteSepTM Human B Cell Enrichment Cocktail (Stemcell Technologies, Vancouver, BC, Canada). .. Genomic DNA was extracted with QIAamp DNA Blood Mini Kit (Qiagen, Venlo, The Netherlands) following the manufacturer’s “Blood or Body Fluid Spin Protocol”, using for final elution 130 μL of Buffer AE.

Article Title: Supporting Information for IL-1 RECEPTOR ANTAGONISM REVEALS A YIN-YANG RELATIONSHIP BETWEEN NF B- AND INTERFERON-SIGNALING IN CHRONIC LYMPHOCYTIC LEUKEMIA
Article Snippet: .. The cells were purified as before (4, 5) by negative selection using RosetteSepTM Human B cell enrichment Cocktail (catalog. no. 15064) from Stemcell technologies (Vancouver, BC). ..

Gradient Centrifugation:

Article Title: Unveiling the dynamics and molecular landscape of a rare chronic lymphocytic leukemia subpopulation driving refractoriness: insights from single-cell RNA sequencing.
Article Snippet: .. B-cells were separated from peripheral blood using the RosetteSepTM Human B Cell Enrichment Cocktail (Stemcell Technologies, Vancouver, Canada) and Ficoll-Paque PLUS (GE Healthcare, Chicago, IL, USA) density gradient centrifugation. ..

Article Title: Development of KoRV-pseudotyped lentiviral vectors for efficient gene transfer into freshly isolated immune cells.
Article Snippet: .. Cells were then isolated by standard density- gradient centrifugation using Ficoll-Paque (VWR, Germany, Catalog. Nr. 17- 1440-03) using the RosetteSepTM Human NK Cell Enrichment Cocktail (Stemcell Technologies, Canada) for NK cells, the RosetteSepTM Human B Cell Enrichment Cocktail for B cells and the RosetteSepTM Human Monocyte Enrichment Cocktail for monocytes, respectively. .. NK cells were cultured at 1 × 106 cells/mL in NK MACS medium (Miltenyi Biotec, Germany) with 5% human AB serum (Sigma Aldrich, USA, Catalog Nr. H4522-100ML), 500 U/mL IL-2 and 140 U/mL IL-15 (Peprotech, USA).

Article Title: Unveiling the dynamics and molecular landscape of a rare chronic lymphocytic leukemia subpopulation driving refractoriness: insights from single‐cell RNA sequencing
Article Snippet: .. B‐cells were separated from peripheral blood using the RosetteSepTM Human B Cell Enrichment Cocktail (Stemcell Technologies, Vancouver, Canada) and Ficoll‐Paque PLUS (GE Healthcare, Chicago, IL, USA) density gradient centrifugation. ..

Centrifugation:

Article Title: Development of KoRV-pseudotyped lentiviral vectors for efficient gene transfer into freshly isolated immune cells
Article Snippet: .. Cells were then isolated by standard density-gradient centrifugation using Ficoll-Paque (VWR, Germany, Catalog. Nr. 17-1440-03) using the RosetteSepTM Human NK Cell Enrichment Cocktail (Stemcell Technologies, Canada) for NK cells, the RosetteSepTM Human B Cell Enrichment Cocktail for B cells and the RosetteSepTM Human Monocyte Enrichment Cocktail for monocytes, respectively. .. NK cells were cultured at 1 × 10 6 cells/mL in NK MACS medium (Miltenyi Biotec, Germany) with 5% human AB serum (Sigma Aldrich, USA, Catalog Nr. H4522-100ML), 500 U/mL IL-2 and 140 U/mL IL-15 (Peprotech, USA).



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